HPLC Columns: A Comprehensive Guide to Selection, Applications, and Leading Manufacturers
Introduction to HPLC Columns High-Performance Liquid Chromatography (HPLC) is a vital analytical technique used across various industries, including pharmaceuticals, biotechnology, food safety, and environmental testing. At the core of every HPLC system lies the HPLC column , the component responsible for the separation of analytes based on their chemical properties. Choosing the right HPLC column is crucial for achieving accurate, reproducible, and efficient results. Understanding HPLC Columns An HPLC column is a tube filled with a stationary phase that interacts with sample components as they pass through under high pressure. The separation process depends on differences in the interaction between the stationary phase and the mobile phase carrying the analytes. The performance of an HPLC column is influenced by factors such as: Particle Size: Smaller particles provide higher resolution but require higher pressure. Pore Size: Determines the molecular weight range of compounds tha...